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bacterial dna  (Bio-Rad)


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    Bio-Rad bacterial dna
    Bacterial Dna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial dna/product/Bio-Rad
    Average 95 stars, based on 282 article reviews
    bacterial dna - by Bioz Stars, 2026-02
    95/100 stars

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    Construction of an M. feriruminatoris ∆ hsdR mutant. ( A ) Schematic representation of the pipeline followed to generate the Δ hsdR mutant of M. feriruminatoris . Isolated gDNA of the WT strain is cotransformed into S. cerevisiae together with the LP recombination template and two plasmids carrying the eSpcas9 gene and the corresponding gRNA. Modified genome is isolated and transplanted into M. capricolum recipient cells to finally isolate the M. feriruminatoris Δ hsdR mutant. ( B ) Transformation efficiency of the WT and Δ hsdR mutant strains with the pIVB09 plasmid. N = 3 biological replicates, *** P < .001. Created partially in BioRender: Torres Puig, S. (2025) https://BioRender.com/oyn7tvl.

    Journal: Nucleic Acids Research

    Article Title: A versatile plug and play bacterial chassis based on a fast-growing Mycoplasma species

    doi: 10.1093/nar/gkaf1134

    Figure Lengend Snippet: Construction of an M. feriruminatoris ∆ hsdR mutant. ( A ) Schematic representation of the pipeline followed to generate the Δ hsdR mutant of M. feriruminatoris . Isolated gDNA of the WT strain is cotransformed into S. cerevisiae together with the LP recombination template and two plasmids carrying the eSpcas9 gene and the corresponding gRNA. Modified genome is isolated and transplanted into M. capricolum recipient cells to finally isolate the M. feriruminatoris Δ hsdR mutant. ( B ) Transformation efficiency of the WT and Δ hsdR mutant strains with the pIVB09 plasmid. N = 3 biological replicates, *** P < .001. Created partially in BioRender: Torres Puig, S. (2025) https://BioRender.com/oyn7tvl.

    Article Snippet: YACs with M. feriruminatoris genomes containing the LP were isolated in agarose plugs using the CHEF yeast genomic DNA plug kit (Bio-Rad) as described elsewhere [ ] and subsequently transplanted into Mcap ΔRE recipient cells as done previously [ , ].

    Techniques: Mutagenesis, Isolation, Modification, Transformation Assay, Plasmid Preparation